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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Amplified Fragment Length Polymorphism Analysis of Campylobacter jejuni and Campylobacter coli from Broiler Farms and Different Processing Stages in Poultry Slaughterhouses ผู้เขียน: เฉลิมเกียรติ แสงทองพินิจ, Sasitorn Kanarat,  ดร.ธีระพล ศิรินฤมิตร, รองศาสตราจารย์ , ดร.ปฐมาพร เอมะวิศิษฎ์, รองศาสตราจารย์ , ดร.ธวัชชัย ศักดิ์ภู่อร่าม, ผู้ช่วยศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe aim of this study was to use the high-resolution genotyping method of amplified fragment length polymorphism (AFLP) to investigate the genetic diversity of Campylobacter jejuni and C. coli isolates obtained from broiler farms and different stages of slaughterhouse processing. Of 490 samples, 328 isolates of Campylobacter spp. were found (328/490, 66.9%) and the isolates were identified as C. jejuni (24.1%, 118/490) and C. coli (42.8%, 210/490). The genetic fingerprint of the 314 Campylobacter isolates, as determinated by AFLP, revealed 48 AFLP strains of C. jejuni and 95 AFLP strains of C. coli. In most flocks, a broad diversity of C. jejuni and C. coli strains was found and the distribution of AFLP types changed during slaughter line processing. Some genotypes were found to be the contamination source of both species in chicken intestines and by direct contamination of chicken meat during the slaughtering and cutting processes in the slaughterhouse. AFLP fingerprinting was an effective method to discriminate between C. jejuni and C. coli strains, in which the interlinkage homology of the AFLP pattern was only 35-42%. In addition, AFLP fingerprinting could distinguish between strains that were genetically unrelated or related. Therefore, AFLP analysis was considered a suitable epidemiological tool for investigation of Campylobacter. |
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